Dr. Thomas Hartwig
Research focus
Variation in regulatory elements is a major cause of phenotypic diversity, yet notoriously difficult to pinpoint. We developed FIND-CIS, a combined transcription factor foot-printing assay (MOA-seq), RNA-seq, and F1 hybrids to quantify functional variation that affects gene regulation. Our aim is to improve biotic and abiotic traits in cops by targeted editing of cis-elements. We also strive to understand the evolution, dynamics, and diversity of cistromes.
Most important publications
- Engelhorn J, Snodgrass SJ, Kok A, Seetharam AS, Schneider M, Kiwit T, Singh A, Banf M, Khaipho-Burch M, Runcie DE, Camargo VS, Torres-Rodriguez JV, Sun G, Stam M, Fiorani F, Schnable JC, Bass HW, Hufford MB, Stich B, Frommer WB, Ross-Ibarra J, Hartwig T (2024) Genetic variation at transcription factor binding sites largely explains phenotypic heritability in maize. bioRxiv. doi: 10.1101/2023.08.08.551183
- Hartwig T, Banf M, Prietsch GP, Zhu JY, Mora-Ramirez I, Schippers JHM, Snodgrass SJ, Seetharam AS, Huettel B, Kolkman JM, Yang J, Engelhorn J, Wang ZY (2023) Hybrid allele-specific ChIP-seq analysis identifies variation in brassinosteroid-responsive transcription factor binding linked to traits in maize. Genome Biol 24(1):108. doi: 10.1186/s13059-023-02909-w.
- Liang Z, Myers ZA, Petrella D, Engelhorn J, Hartwig T, Springer NM (2022) Mapping responsive genomic elements to heat stress in a maize diversity panel. Genome Biol 23(1):234. doi: 10.1186/s13059-022-02807-7.
- Savadel SD, Hartwig T, Turpin ZM, Vera DL, Lung PY, Sui X, Blank M, Frommer WB, Dennis JH, Zhang J, Bass HW (2021) The native cistrome and sequence motif families of the maize ear. PLoS Genet 17(8):e1009689. doi: 10.1371/journal.pgen.1009689.
- Hartwig T, Corvalan C, Best NB, Budka JS, Zhu JY, Choe S, Schulz B (2012) Propiconazole is a specific and accessible brassinosteroid (BR) biosynthesis inhibitor for Arabidopsis and maize. PLoS One 7(5):e36625. doi: 10.1371/journal.pone.0036625.
- Hartwig T, Chuck GS, Fujioka S, Klempien A, Weizbauer R, Potluri DP, Choe S, Johal GS, Schulz B (2011) Brassinosteroid control of sex determination in maize. Proc Natl Acad Sci U S A 108(49):19814-19819. doi: 10.1073/pnas.1108359108.
ORCID: 0000-0002-2707-2771