Prof. Dr. Lutz Schmitt

Research focus

In plants, ABC transporters are involved in many vital processes such as detoxification or the export of secondary metabolites. However, in vitro data on plant ABC transporters is rather limited, because hardly any system has been successfully overexpressed and purified in quantities and homogeneity suitable for biochemical, biophysical or structural studies. Research in our laboratory aims to understand structural, dynamic and functional aspects of ABC transporters on a molecular level. Therefore, we have selected a minimal set of plant ABC transporters that will be studied employing various in vitro approaches.

The ten most important publications

  1. Lenders MH, Weidtkamp-Peters S, Kleinschrodt D, Jaeger KE, Smits SH, Schmitt L (2015) Directionality of substrate translocation of the hemolysin A Type I secretion system. Scientific reports 5:12470.
  2. Gupta RP, Kueppers P, Hanekop N, Schmitt L (2014) Generating symmetry in the asymmetric ABC transporter Pdr5 from Saccharomyces cerevisiae. The Journal of biological chemistry 289:15272- 15279.
  3. Gawarzewski I, DiMaio F, Winterer E, Tschapek B, Smits SH, Jose J, Schmitt L (2014) Crystal structure of the transport unit of the autotransporter adhesin involved in diffuse adherence from Escherichia coli. J Struct Biol 187(1):20-29.
  4. Lecher J, Schwarz CK, Stoldt M, Smits SH, Willbold D, Schmitt L (2012) An RTX transporter tethers its unfolded substrate during secretion via a unique N-terminal domain. Structure 20(10):1778-1787.
  5. Tschapek B, Pittelkow M, Sohn-Bosser L, Holtmann G, Smits SH, Gohlke H, Bremer E, Schmitt L (2011) Arg149 is involved in switching the low affinity, open state of the binding protein AfProX into its high affinity, closed state. J Mol Biol 411(1):36-52.
  6. Bakkes PJ, Jenewein S, Smits SH, Holland IB, Schmitt L (2010) The rate of folding dictates substrate secretion by the Escherichia coli hemolysin type 1 secretion system. The Journal of biological chemistry 285(52):40573-40580.
  7. Oswald C, Smits SH, Hoing M, Sohn-Bosser L, Dupont L, Le Rudulier D, Schmitt L, Bremer E (2008) Crystal structures of the choline/acetylcholine substrate-binding protein ChoX from Sinorhizobium meliloti in the liganded and unliganded-closed states. The Journal of biological chemistry 283(47):32848-32859.
  8. Ernst R, Kueppers P, Klein CM, Schwarzmueller T, Kuchler K, Schmitt L (2008) A mutation of the H-loop selectively affects rhodamine transport by the yeast multidrug ABC transporter Pdr5. Proc Natl Acad Sci U S A 105(13):5069-5074.
  9. Zaitseva J, Oswald C, Jumpertz T, Jenewein S, Wiedenmann A, Holland IB, Schmitt L (2006) A structural analysis of asymmetry required for catalytic activity of an ABC-ATPase domain dimer. Embo J 25(14):3432-3443.
  10. Zaitseva J, Jenewein S, Jumpertz T, Holland IB, Schmitt L (2005) H662 is the linchpin of ATP hydrolysis in the nucleotide-binding domain of the ABC transporter HlyB. Embo J 24:1901-1910.
Prof. Dr. Lutz Schmitt
CEPLAS Lutz Schmitt

lutz.schmitt[at]hhu.de

 +49 211 81-10773

Institute of Biochemistry I
Heinrich Heine University Düsseldorf

Universitätsstraße 1

40225 Düsseldorf

www.biochemistry1.hhu.de/en.html

Heinrich Heine University
University of Cologne
Max Planck Institute for Plant Breeding Research
Forschungszentrum Jülich